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SIRT-1在不同宫颈组织中的表达及其临床意义
张惠文;马德花;张磊;赵淑萍;
0
(青岛市妇女儿童医院; 青岛大学附属医院)
摘要:
目的:探讨Ⅲ型去乙酰化酶SIRTUIN1(SIRT-1)蛋白在正常宫颈组织及不同级别宫颈病变组织中的表达及其临床意义。方法:应用Western-blot方法及免疫组化(SP)法检测正常宫颈组织(24例)、低级别鳞状上皮内病变组织(LSIL)26例、高级别鳞状上皮内病变组织(HSIL)35例及宫颈癌组织55例中SIRT-1蛋白的表达情况;并分析SIRT-1蛋白的表达与宫颈癌组织各临床病理参数间的关系。结果:(1)SIRT-1蛋白的高表达率在正常宫颈组织为0、LSIL为15.38%、HSIL为37.14%及宫颈癌组织为40.00%,差异有统计学意义(χ~2=16.82,P<0.05);(2)宫颈癌组织中SIRT-1蛋白的高表达率在不同组织学分化程度(G1~G3)(χ~2=6.87,P<0.05)、有无淋巴结转移(χ~2=7.73,P<0.05)、不同临床分期(χ~2=7.39,P<0.05)间差异均有统计学意义。结论:SIRT-1随宫颈病变的加重表达逐渐增强,与宫颈癌组织学分化程度、有无淋巴结转移、临床分期有关。可能在宫颈癌的发生和发展过程中起促进作用。
关键词:  Ⅲ型去乙酰化酶SIRTUIN1  免疫组化  宫颈病变  宫颈癌
DOI:
基金项目:山东省自然科学基金(编号:ZR2015HM035)
Expressions and Clinical Significance of SIRT-1 in Normal Tissue and Different Staging Lessions of Cervix
ZHANG Huiwen;MA Dehua;ZHANG Lei;ZHAO Shuping;
(Qingdao Women and Children’s Hospital;The Affiliated Hospital of Qingdao University)
Abstract:
Objective: To investigate the expressions of SIRT-1 protein in normal cervical tissue and different cervical lessions and it’ s clinical significance. Methods: Using western-blot method and immunohistochemical method( SP) to detect SIRT-1 protein expression level in normal cervical tissue( 24 cases) 、low-grade squalors intraepithelial lesion( 26 cases),the high-grade squalors intraepithelial lesion( 35 cases) and cervical cancer group( 55 cases); and to discuss the relationship between clinical pathological parameters with cervical cancer tissue. Results:(1)The positive expression rate of SIRT-1 protein in normal cervical tissues,LSIL,HSIL and cervical cancer tissues were 0. 00%,15. 38%,37. 14%,40. 00% respectively,the difference was statistically significant( χ~2= 16. 82,P <0. 05),and the trend test was statistically significant too;(2)Cervical cancer tissues SIRT-1 protein positive expression rate in different histological differentiation degree( G1-G3) was statistically significant( χ~2=6. 87,P<0. 05); the difference between lymph node metastasis group and lymph node metastasis-negative group was significantly different( χ~2=7. 73,P<0. 05),and is associated with clinical stage( χ~2= 7. 39,P <0. 05); the protein expression rate of SIRT-1 was not significant correlated with the histopathological types( χ~2= 1. 03,P>0. 05). Conclusions: The expression of SIRT-1 gradually strengthen with the deterioration of the cervical lesion,and cervical cancer histological differentiation degree,with or without lymph node metastasis and clinical stage. The over-expression of SIRT-1 may play a promote role in cervical cancer occurrence and development process
Key words:  Sirtuintype1  Immunohistochemistry  CervicalLesions  Cervicalcancer

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